Study of activity transcription factors C/EBPα in region - 53 to - 33 of promoter apolipoprotein B gene

Apolipoproteins are important constituents of lipoprotein particles and play a central role in the transport and metabolism of lipids (Goldstein et al., 1983). Different lipoproteins and apo have been identified. Recent epidemiological studies have shown the importance of the apolipoprotein B, and its direct correlation with the incidence of coronary heart disease (Sattar et al., 2004; Olofsson, Borén, 2005; Barter et al., 2006; Thompson, Danesh, 2006). Apolipoprotein B (apo B) is a constituent of several lipoproteins and acts as a ligand for cell recognition and catabolism of low density lipoprotein (LDL) by the LDL receptor (Goldstein, Brown 1977; Brown, Goldstein, 1986). It is the structural protein moiety of plasma low density lipoprotein (LDL), comprising 23.8% of the LDL particle (Goldstein, Brown, 1983). Thus, it is reasonable to expect that changes in the regulation of apolipoprotein B synthesis will affect the plasma lipids or lipoprotein profiles and contribute to the pathogenesis of coronary heart disease (Grundy, 2005; Barter et al., 2006). Apo B gene is localized in chromosome 2pter-2p24 and comprises 29 exons and 28 introns (Huang, Manson, 1986), and it is expressed in the liver, intestine and placenta, showing that ApoB gene transcription is regulated in a tissuespecific manner (Zannis et al., 2001). In humans, there are two forms of apolipoprotein B, namely apoB-100 and apoB-48. Apolipoprotein B-100 is expressed in the liver and is the main protein part of low density lipoprotein. In contrast, Apo B-48 is synthesized in the small intestine and is a constituent of chylomicrons and their remnants (Breslow, 1988; Lilja et al., 1999; Zannis et al., 2001). Hepatic apoB gene transcription is regulated by several *Correspondence: